Could it be? Is spring on its way? After a lot of working in the last this past February and March, it sure sounds nice to be able to work outside again in the near future!
A killdeer has built its nest on the green roof at the Chicago Botanic Garden. This has happened every year and it’s nice to see a sign that spring is here once again!
Just a few of the many test tubes filled with plant DNA that I’ve been working with the past two months.
The winter to spring transition months included a lot of test tubes! I’m happy to report that I finally finished extracting the DNA from all of my little seedlings. That’s almost 550 samples. Phew! It took a lot longer than I expected just to perfect the technique of getting DNA out of such little bits of plant tissue but I was able to get the procedure streamlined enough and finally finished. The next step was to start the DNA amplification – a process called PCR that makes many copies of the DNA so I can work with it in the future. I need to amplify 9 sections of DNA in each of my 550 samples. If you’re doing the math at home, that’s nearly 5,000 reactions. Luckily, there is a machine that helps me out with making temperature changes so the reactions can occur without my constant guidance but I still have the fun task of loading the test tubes with the correct materials – yep, all 5000 combinations. So that’s been most of my March and the project will continue into the future. By the end of the month I was able to test some of the PCR samples and see if they worked. I’m happy to say that I’ve got mostly positive results so far. There are still a few kinks to be worked out but at least I know that things are moving forward in the right direction.
The 2015 seeds have germinated. The seedlings are in these small tubes and are kept frozen until I can find the time to extract their DNA this spring.
Moving in the right direction is a good thing, especially in light of the fact that I’ve got another round of DNA extraction and amplification to go. I’ve just completed these steps with the seeds I collected at the end of the 2014 season. In March, I also collected the germinated seedlings from all of the 2015 seeds. The new little seedlings (only about half as many this time, thank goodness!) had finished getting as big as they were going to get in the incubators so I collected them in small test tubes and put them in a very cold freezer. In April or May I’ll start the DNA extraction procedure all over again with these new samples. Then more amplification…
Things are looking pretty dormant in my green roof plots. But I know my plants are there. Just wait a few months!
As March came to a close, I ventured out to a couple of my roofs just to see if there were any signs of life. It was a pretty mild winter but it still looks too early for most of my little plants to start growing yet. I guess I was just getting a little hopeful – wishful thinking! I’m looking forward to getting out to all of my green roof sights again this spring and summer. It’s hard to believe (but kind of exciting too) that this will be the last summer of data collection for my dissertation research. In the future, I’m not sure what will happen to these plots that I’ve established, but I think at least some of them will be left alone and the plants will just do what plants do; grow, reproduce… hopefully survive for many generations. We’ll have to see. One thing I do know is that it’s going to be a busy summer.
By the end of March, a few signs of life started to appear in my green roof plots.
My temperature probes (like this one taped to the roof) have been recording data all winter long (hopefully). I’ll collect them again this spring to see what happened on the roofs while I was inside staying warm.
In other fun news, my green roof children’s activity book has been featured, both on an industry website and in a non-profit magazine. My coauthor Olyssa and I were asked to write a little piece describing the unique features of our book for the international website greenroofs.com. Check out that story by clicking here. I was also interviewed by a reporter a couple months back (remember that photo shoot in December that I wrote about in my last post?) about the environmental education benefits of our book and a short piece was included in the Chicago Botanic Garden’s member magazine, Keep Growing. Check out that article by clicking here and going to page 74. We continue to have people download our free book and have recently even been asked to translate it into Dutch for a wider international audience. It’s great to know that people are enjoying the book and that our hard work is helping teach people about the benefits of green roofs!